Links to detailed protocols and methods are provided below.
Plant Tissue Culture & Transformation
Hybrid poplar NM6 transformation
A simple improved-throughput xylem protoplast system for studying wood formation (Nature protocols 2014 9: 2194-2205)
Transcript Analysis
Total RNA extraction (not for analysis of small RNA)
qRT-PCR (RNAs including mRNA and 18S rRNA)
Protein Analysis
P. trichocarpa crude protein extraction
Plant total protein microsomal fraction isolation
Protein expression and purification for yeast
GST fusion protein expression and purification
Histag protein expression and purification
Protein & DNA Interaction Analysis
Electrophoretic moblility shift assay
A robust chromatin immunoprecipitation protocol for studying transcription factor-DNA interactions and histone modifications in wood-forming tissue (Nature protocols 2014 9: 2180-2193)
Metabolite
Metabolite isolation from developing xylem
Organic and biochemical synthesis of monolignol biosynthetic pathway intermediates
Milled wood lignin isolation for NMR
Wood Chemistry
Cytology
Histochemistry of tree stem section
Laser microdissection of xylem tissue
Bioinformatics Analysis