| Command | Followed by | Means |
| -filetype | qtls.inp | Tells Rqtl what to expect in the format. |
| -Units | cM or M | cM for centiMorgans and M for Morgans. |
| -named | yes or no | Answers whether the traits will have names. |
| -start | qtls | Begin data defining QTLs |
| -interactions | Begin data on defining interactions | |
| -skip | Tells Rqtl to skip everything until an | |
| -unskip | which is the end of the -skip. These | |
| cannot be within a -start to -stop block | ||
| or a -interactions to -stop block | ||
| -stop | End of a qtls or interactions block | |
| -end | End of the file |