This page contains results of the qRT-PCR that were used for first round of screening for transgenic tree of different lines of the same construct, and was the basis for selecting three to four transgenic lines with different levels of target gene knockdown. The data are organized by constructs or genes. Click on any of the constructs or genes to see the qRT-PCR result for transgenic lines associated with that construct or gene. For primers used in the qRT-PCR please refer to Shi et al. (2010). If you have any questions about these data, would like to use these data for publication, or would like to collaborate, please contact Vincent Chiang (Contacts Page).
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Results by Construct
Family | Construct | Gene |
---|---|---|
4CL | a12 | 4CL3 |
4CL5 | ||
a13 | 4CL3 | |
4CL5 | ||
i15 | 4CL3 | |
4CL5 | ||
wt | 4CL3 | |
4CL5 | ||
CAld5H | a27 | CAld5H1 |
CAld5H2 | ||
a28 | CAld5H1 | |
CAld5H2 | ||
i29 | CAld5H1 | |
CAld5H2 | ||
wt | CAld5H1 | |
CAld5H2 | ||
LIM | a40 | LIM1 |
a41 | LIM2 | |
i42 | LIM1 | |
LIM2 | ||
wt | LIM1 | |
LIM2 | ||
MYB | a43 | MYB167 |
a44 | MYB090 | |
i45 | MYB090 | |
MYB167 | ||
PAL | a1 | PAL1 |
PAL3 | ||
a2 | PAL3 | |
a3 | PAL4 | |
a4 | PAL5 | |
a5 | PAL2 | |
i6 | PAL1 | |
PAL3 | ||
i7 | PAL2 | |
PAL4 | ||
PAL5 | ||
i8 | PAL1 | |
PAL2 | ||
PAL3 | ||
PAL4 | ||
PAL5 | ||
wt | PAL1 | |
PAL2 | ||
PAL3 | ||
PAL4 | ||
PAL5 |
References
Shi, R., et al. 2010. Plant Cell Physiol. 50:144-163.